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Image Search Results
Journal: Toxicological Sciences
Article Title: From the Cover: Three-Dimensional (3D) HepaRG Spheroid Model With Physiologically Relevant Xenobiotic Metabolism Competence and Hepatocyte Functionality for Liver Toxicity Screening
doi: 10.1093/toxsci/kfx122
Figure Lengend Snippet: CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen (CYP1A2); 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Article Snippet: TaqMan assay probes (
Techniques: Activity Assay, Liquid Chromatography with Mass Spectroscopy, Cell Culture
Journal: International Journal of Molecular Sciences
Article Title: The Autotaxin-LPA Axis Emerges as a Novel Regulator of Smooth Muscle Cell Phenotypic Modulation during Intimal Hyperplasia
doi: 10.3390/ijms24032913
Figure Lengend Snippet: Treatment with the ATX inhibitor rescues mRNA encoding proteins associated with inflammation following injury. ( A ) Chemokine and cytokine mRNA expression was measured on day 3 with and without PF8380 treatment following injury. ( B ) Transcriptional mRNA expression levels of IL-6 with and without PF8380 treatment for different time points. ( C ) Representative sections of mouse carotid artery stained with CD68 antibody (C68-target for macrophages) with and without PF8380 treatment at 2 weeks after injury. Scale bar = 50 μm. ( D ) Superoxide measured using high-performance liquid chromatography in uninjured, injured, and PF8380-treated mice at day 14. All values are mean ± SD ( n = 6) compared to the uninjured vessel using the Mann–Whitney U test.
Article Snippet: The primers used were
Techniques: Expressing, Staining, High Performance Liquid Chromatography, MANN-WHITNEY