gencap ® liquid phase target gene capture Search Results


99
Thermo Fisher gene exp cyp1a2 hs00167927 m1
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Gene Exp Cyp1a2 Hs00167927 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MyGenostics Inc gencap liquid-phase target gene capture
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Gencap Liquid Phase Target Gene Capture, supplied by MyGenostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AusDiagnostics Pty Ltd gene-plex cas1212 liquid handling robot
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Gene Plex Cas1212 Liquid Handling Robot, supplied by AusDiagnostics Pty Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DaAn Gene diaminobenzidine chromogenic liquid
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Diaminobenzidine Chromogenic Liquid, supplied by DaAn Gene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SeraCare Life Sciences 520 gene liquid biopsy oncocompass plus
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
520 Gene Liquid Biopsy Oncocompass Plus, supplied by SeraCare Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DaAn Gene 3,3′-diaminobenzidine chromogenic liquid
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
3,3′ Diaminobenzidine Chromogenic Liquid, supplied by DaAn Gene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nihon Gene Research Laboratories hplc purified rnas and dnas
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Hplc Purified Rnas And Dnas, supplied by Nihon Gene Research Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioWindow Gene Development Inc inorganic nanoshell-stabilized liquid metal
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Inorganic Nanoshell Stabilized Liquid Metal, supplied by BioWindow Gene Development Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Gene Design Inc hplc
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Hplc, supplied by Gene Design Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Johns Hopkins HealthCare foundationone liquid cdx
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Foundationone Liquid Cdx, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iCell Gene Therapeutics cryopreserved vials of icell human cardiac myocytes
CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen <t>(CYP1A2);</t> 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.
Cryopreserved Vials Of Icell Human Cardiac Myocytes, supplied by iCell Gene Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Thermo Fisher gene exp il6 mm99999064 m1
Treatment with the ATX inhibitor rescues mRNA encoding proteins associated with inflammation following injury. ( A ) Chemokine and cytokine mRNA expression was measured on day 3 with and without PF8380 treatment following injury. ( B ) Transcriptional mRNA expression levels <t>of</t> <t>IL-6</t> with and without PF8380 treatment for different time points. ( C ) Representative sections of mouse carotid artery stained with CD68 antibody (C68-target for macrophages) with and without PF8380 treatment at 2 weeks after injury. Scale bar = 50 μm. ( D ) Superoxide measured using high-performance liquid chromatography in uninjured, injured, and PF8380-treated mice at day 14. All values are mean ± SD ( n = 6) compared to the uninjured vessel using the Mann–Whitney U test.
Gene Exp Il6 Mm99999064 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen (CYP1A2); 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.

Journal: Toxicological Sciences

Article Title: From the Cover: Three-Dimensional (3D) HepaRG Spheroid Model With Physiologically Relevant Xenobiotic Metabolism Competence and Hepatocyte Functionality for Liver Toxicity Screening

doi: 10.1093/toxsci/kfx122

Figure Lengend Snippet: CYP450 enzyme activity in 2D HepaRG cells, HepaRG spheroids and primary human hepatocytes. LC-MS/MS analysis of metabolite formation rate of acetaminophen (CYP1A2); 1-hydroxybupropion (CYP2B6) and 1-hydroxymidazolam (CYP3A4). Data were the mean of three independent experiments (± SEM) from 10-day sandwich-cultured 2D HepaRG cells and 21-day cultured 3D HepaRG spheroids. Data from primary suspension cultures were from 212, 137 and 132 individual donor preparations for CYP1A2, CYP2B6 and CY3A4/5, respectively, and 52 individual donor preparations for 4-day sandwich–cultured primary hepatocytes.

Article Snippet: TaqMan assay probes (ThermoFisher, Waltham, MA) used are Hs04183483_g1 (CYP2B6), Hs00604506_m1 (CYP3A4), Hs02758991_g1 (GAPDH), Hs00167927_m1 (CYP1A2). mRNA levels of target genes were normalized to housekeeping gene GAPDH.

Techniques: Activity Assay, Liquid Chromatography with Mass Spectroscopy, Cell Culture

Treatment with the ATX inhibitor rescues mRNA encoding proteins associated with inflammation following injury. ( A ) Chemokine and cytokine mRNA expression was measured on day 3 with and without PF8380 treatment following injury. ( B ) Transcriptional mRNA expression levels of IL-6 with and without PF8380 treatment for different time points. ( C ) Representative sections of mouse carotid artery stained with CD68 antibody (C68-target for macrophages) with and without PF8380 treatment at 2 weeks after injury. Scale bar = 50 μm. ( D ) Superoxide measured using high-performance liquid chromatography in uninjured, injured, and PF8380-treated mice at day 14. All values are mean ± SD ( n = 6) compared to the uninjured vessel using the Mann–Whitney U test.

Journal: International Journal of Molecular Sciences

Article Title: The Autotaxin-LPA Axis Emerges as a Novel Regulator of Smooth Muscle Cell Phenotypic Modulation during Intimal Hyperplasia

doi: 10.3390/ijms24032913

Figure Lengend Snippet: Treatment with the ATX inhibitor rescues mRNA encoding proteins associated with inflammation following injury. ( A ) Chemokine and cytokine mRNA expression was measured on day 3 with and without PF8380 treatment following injury. ( B ) Transcriptional mRNA expression levels of IL-6 with and without PF8380 treatment for different time points. ( C ) Representative sections of mouse carotid artery stained with CD68 antibody (C68-target for macrophages) with and without PF8380 treatment at 2 weeks after injury. Scale bar = 50 μm. ( D ) Superoxide measured using high-performance liquid chromatography in uninjured, injured, and PF8380-treated mice at day 14. All values are mean ± SD ( n = 6) compared to the uninjured vessel using the Mann–Whitney U test.

Article Snippet: The primers used were Mm99999064_m1 ( IL6 ), Mm01200332_g1 ( Enpp2 ), Mm01346925_m1 ( LPAR1 ), Mm00469562_m1 ( LPAR2 ), Mm00469694_m1 ( LPAR3 ), Mm02620784_s1 ( LPAR4 ), Mm02621109_s1 ( LPAR5 ), Mm00725412_s1 ( Acta2 ), Mm00487032_m1 ( Cnn1 ), Mm00441661_g1 ( Tagln ), Mm00443013_m1 ( Myh11 ), and Mm99999915_g1 ( GAPDH ), which were obtained from Applied Biosystems (Foster City, CA, USA).

Techniques: Expressing, Staining, High Performance Liquid Chromatography, MANN-WHITNEY